An adult character in Anor Londo is wearing a very form fitting and revealing dress, however they do not seem to be distressed about this and chose to wear it/be there. Players may leave comments such as “Amazing chest ahead”, but that is the extent and can be avoided by playing offline.
Depending on whether one NPC's quest is followed, there is a point where they offer to sacrifice their life by distracting a horde of enemies so that the player may pass through. They don't have to die if the player fights with them, but if the player chooses to run through and leave them behind that will be the end of the character.
"We are amidst strange beings, in a strange land. The flow of time itself is convoluted, with heroes centuries old phasing in and out. The very fabric wavers, and relations shift and obscure. There's no telling how much longer your world and mine will remain in contact."
Many weapons from bosses can lay the player out on the ground if hit, may evoke the same emotion. One boss in particular uses an attack where he slams his massive hand on the ground.
A character described as male , Gwyndolin, is raised as female against his will for being effeminate. It is implied that he is his father's least favorite child because of his lack of masculinity.
Unless you count the player characters need to constantly chug glowing orange juice, no.
There are various items that can be consumed but I wouldn't consider any of them drugs, they are mostly buff and healing items.
There are several (very, very large) cats that are typically fought and killed in one area of the game, though they are not strictly mandatory to fight and could theoretically be skipped.
There are a handful of dragon and dragonlike enemies that can be killed, including two bosses. One of the dragon bosses must be killed to complete the game.
There is a boss called Quelaag who is half spider (her bottom half is a giant spider). Her sister who is also half spider is an npc.
There are spider-like enemies in the same area though they can be avoided and aren't particularly bad.
There is an enemy phantom who is named man eater Mildred who is canonically a cannibal and there are butchers in the game who are cutting in identified meat, but no eating of humans is shown
There are many points where you can be burned alive, and if you choose to you can burn others alive. While such victims clearly emote that they are suffering, the effect is not particularly graphic.
In order to join a specific covenant the player must “nestle into” a coffin in an underground crypt. This is optional and the player character is able to harmlessly leave the coffin after about 30 seconds.
Individuals are abducted by Seath's various minions, but they are not children and it's very easy to play the game without noticing this/figuring it out.
Sort of. There aren't any scripted jumpscares, but there are definitely times when an enemy can sneak up on you and frighten you or attack from off screen. There are also enemies that hide and can grab you unawares. It's not particularly offensive, and it isn't really a horror game. There are definitely scary parts though. There is an area that is very dark and you are required to carry a light source, even when you do you can only see a short distance and enemies can and likely will appear out of darkness and scare you.
Some nonhuman enemies have spitting attacks. As well as attacks that cause clouds of gas, no farting that I know of.
There is a throwable item called "dung pie" which is poop.
There is also an opportunity to perform a critical attack on a boar where the player stabs a sword up it's bottom.
There’s an option to climb inside a stone casket. It closes on you, and there’s a short stretch where you see from the inside where you’re trapped. This only lasts a few seconds and might be skippable (don’t remember off the top of my head).
As mentioned above (does a mother die) this is only implied by a "twin souls" item being recovered from a woman's corpse. It's also missable and skippable.
Gwyndolin, who is arguably a trans woman, is referred to with male pronouns. In-game messages left by other players frequently call Gwyndolin the t-slur, although this was not the choice of the developers.
Gwyndolin is described in-game as a boy raised as a girl, although it is implied that they may be a trans woman. Either way, they are consistently misgendered.
Queelag is a half-woman, half-spider hybrid, and her torso is nude, however, her chest is covered by her hair. Also, players can leave messages in your world, and a possible message is "Try finger but hole". I think those are the only sexual parts.
The main character either ends up linking the first flame, revitalizing the land and curing the undead curse at the cost of their life, or to not kindle the flame, and bring about an age of darkness.