Jack has a tendency to view women more as objects. There are scenes where he'll override your scanning device and rate the female members of your team out of ten, advising on whether you should have sex with them or not. The more you enable and cooperate with Jack, the more you see him make comments like this
Fiona and Sasha's adoptive father-figure betrays the group in the first episode, leaving the sisters distraught for several episodes. While both sisters are adults and it is later revealed to have been done for their safety, it's still a lengthy point of tension.
Yes, once for comedy and once more seriously. The character lives both times. Another character’s death could be interpreted as a sacrifice, but they would have died regardless.
Fiona's father figure can die depending on the player's choices - this is heavily telegraphed and easy to avoid. August's mother always dies. Scooter dies, though the rest of his family makes no appearance in this game. Sasha the kid sister always survives.
Implied - Handsome Jack claims that a character used to run a 'kitten fighting ring', which involved said character beating kittens. Whether or not this is true is never confirmed.
Two mandatory QTE segments involve the player removing eyeballs from still-living characters. One mandatory segment where the player must remove their own (cybernetic) arm while fully conscious. An optional instance where the player can shoot a character's arm off. (...Also, uh, a corpse's whole face gets amputated, and you have to steal it.)
A character falls from a substantial height, which prompts another character to declare "Oh yeah, that's broken." The injury doesn't break the skin and it's portrayed for comedy.
A major character is stabbed in episode 4 as a threat to the rest of the cast. This is an optional scene and can be avoided by selecting the "you win" dialogue option when prompted.
A scene requires the player to input commands to remove a dead character's eyeball to proceed. It cannot be avoided, and the eyeball itself is used again in a later episode.
While technically not a ghost, the player is followed around by a holographic AI of his dead boss that only he can see and hear. He's intangible and capable of possessing the protagonist, so the distinction is pretty thin.
Rhys throws up during the opening sequence in episode 4. He has a tendency to gag or retch at gruesome scenes and references to vomiting are made in episodes 3 and 4, but there's only one instance depicted onscreen.
No, but there are scenes where a character is shocked (most likely in their brain) by a malfunctioning/shady device that they plug into a cybernetic port on their temple. They seize up and pass out, and it is played for laughs. Characters are also shocked/electrocuted, with Jack comparing it to electroshock therapy, and then making a comment that he wished he'd used electroshock therapy on his ex-girlfriend.
in the final episode, rhys tears out his own cybernetic arm and eye, which causes him considerable pain and which you have to guide him through doing with QTEs.
A larger woman threatens you with a massive rocket launcher, and you can make a comment about her weight. "I don't know what to be more afraid of, the oversized piece of crap that belches fire, or the rocket launcher she's holding."
In one of the scenes in Episode 3 or 4 (I dont remember which), Rhys, a male character, is called "madam" by Loader Bot. Although he is not explicitly stated as trans, it is still worth mentioning.
Image example here: https://imgur.com/5CXHkTQ.png
In the realm of sexism, yes. Jack is portrayed as (among other things) flagrantly sexist. At one point he agrees with something a female character says and responds, while laughing, "She's right! She's a woman and she's right!"
Residents of Pandora wearing masks are frequently referred to as psychos. These characters are depicted as bloodthirsty and barely capable of coherent speech (almost exclusively communicating in violent nonsequiturs)