The game is extremely violent and several characters die regardless of gender. Women are not specifically singled out, but the overall atmosphere is grim and brutal. Gender, femininity, menstruation, and pregnancy are all major underlying themes. Potentially triggering events (spoilers):
-The Queen of Yharnam is depicted with her wrists bound and wearing a wedding dress. She is pregnant and covered in blood near her womb.
-A female character is a prostitute. If she survives until the game's third act, she gives birth to an eldritch creature.
Father Gascoigne goes mad during the Hunt, and his wife leaves her daughter to find him. You can speak to her through the window at her house, and she asks you to find her parents.
There's several eldritch alien babies you can kill, though they don't really look like babies.
Especially not the Orphan of Kos, who is newborn but also looks like an old man.
The boss Rom the Vacuous Spider is a huge, spider-like Great Old One. She also periodically summons a horde of spiders. These appear as regular enemies in the Nightmare of Mensis area.
Women are unwillingly impregnated by Eldritch Abominations. This usually happens by magic shennanigans instead of direct assault, but I'd say unwilling impregnation counts as sexual assault.
One version of the bloodletting beast, found in the Lower Pthumeru Chalice (Layer 4) and the Great Pthumeru Ihyll Chalice (Layer 2) is headless, and the muscle inside of its neck is visible.
If the player is killed by a certain enemy during a certain part of the game, it is implied that they are actually knocked unconscious. During a scene from the player's POV, they awaken while being dragged in a cloth bag to a new area.
Beasts with multiple/too many eyes are fought commonly late in the game, and though there is no detailed eye gore, this can still be upsetting to somebody with trypophobia or a fear of things with too many eyes.
Father Gasçoine's daughter Violet can be eaten by a giant pig or be tortured to death in medical experiments, depending on player choice (offscreen). When her sister Adelle finds out about this, she jumps to her death.
The final boss is an undead stillborn Great Old One and its nurse.
(Old Hunters DLC) The boss Orphan of Kos is a newborn eldritch abomination that must be fought and killed
The first boss is a preist by the name of Father Gasçoine, who you meet after he has succumbed to the Scourge and killed his wife. He has two daughters, Violet and Adelle.
The entire game is triggering. The whole of the what area is teeth itchingly bad but there are a dozen or more monsters and sequences that either rub up against trypophobia or outright are there to trigger. Miyazaki gravitated towards trypophobia triggers for squick factor in all of his games.
You may come across an npc eating corpses in the forbidden woods. Also in the fishing hamlet there are giant whale enemies that have an attack which will swallow your character whole.
Hoo boy, strap in everyone...
Most enemies are mutated by the Scourge, a werewolf epidemic. One variant of werewolf is mutated to the point that it has exposed, rotten organs and a lower leg replacing its tail.
The Cramped Casket enemy is an appartment buildings worth of corpses all smooshed together from stuffing them into the same coffin and leaving them to rot in the street.
One enemy has its head mounted sideways and is infested with tapeworms.
The boss Brain of Mensis is a giant, undead brain studded with eyes.
On that note, "insight" is implied to grow eyes on the surface of the PC's brain. It's a pun you see...
The boss The One Reborn is a heap of fused, undead corpses used in a failed ritual intended to elevate mankind into Godhood, but resulted in, well, a heap of fused, undead corpses.
One boss is fought 3 times, becoming ever more decrepit as you do so. In the last fight, it's head has fallen off and been replaced by a parasitic worm.
More than an attempt, if Father Gascoigne's daughter is sent to either the Clinic or the Chapel, her older sister will throw herself from a ledge when she finds out her family is all dead.
The sounds from the blood and gore could trigger someone and I think some enemies can be heard eating corpses? There's also an enemy with a machine gun that makes a really grating noise
In the Old Hunter's DLC, a character named Lady Maria of the Astral Clocktower is shown slumped in a chair with a slit throat, though she does grab your wrist a begin speaking to you when you approach her. As you find her in an alternate plane of reality where several confirmed dead characters are "alive" and trapped as a form of punishment, it is heavily implied she killed herself only to become trapped also (possibly intentionally).
The chalice dungeons tend to be closed in and, in some places, tightly packed. Fortunately, the dungeons are entirely avoidable and are used mostly for grinding.
Well, the Old Ones' inability to bear or beget viable offspring is a huge plot point, and the final boss is the undead remains of their latest attempt.
Not technically a peroson, but Kos, an Eldritch extraplaner being known as a "Great One," died for unknown reasons while pregnant, leading to a postmortem birth.
Always off-screen but Arianna gives birth to an Eldritch baby if she survives long enough, and it is implied several women in the game get pregnant, including one you run into two or three times as an "apparition" of sorts.
Weeeeellllll... the baby is a perfectly healthy starborne horror whose birth drove their mother gigglingly bonkers, but it's not a miscarriage... it is, however, vastly accelerated, taking about 6 hours from conception to birth.
"Fatty" is one of the words players can use to write messages, and can frequently be found next to passages that are too narrow for the player's character to enter.
A character who is an old man in present day is shown to have had inappropriate obsessive feelings, most likely of a romantic nature, for a character who was his student when she was a young adult. Given that she has been dead for many years and he has an unnaturally prolonged lifespan, we do not know their relative ages during the period of their (one-sided) "relationship", but the visual ages of the character models could be seen as having a substantial age difference.
A substantial part of the game surrounds a ministration called The Healing Church, with many themes and titles referencing a traditional Christian church
Blood and gore are big plot elements. Blood from wounds, blood that heals, blood that freezes into jewels, blood that never clots offered in sacrifice.