The enslavement of an alien race by humans is a major plot point. Some remarks made by human characters (particularly in the supplementary comics) echo real-world hate speech.
The "Human Experimentation" chapter traps the cast in a cave with a deadly disease. They manufacture a cure by experimenting on each other. Vitals are monitored and injections given.
The bonus DLC chapter suggests that the "gods" in the title are the players. Characters refer to the player by their Steam username (or computer name) and also make reference to the requirements of Steam achievements.
Not in the conventional sense. Characters who die in a given playthrough reappear in later chapters, and have a tendency to flicker and glitch. They are considered alive, and only the player character acknowledges that something is wrong.