there is a minigame involving flies, although you do not have to do that minigame i believe

one of the characters has an alcoholic father

Gin's father is mentioned to be an alcoholic.

In the newest chapter of the game, there is a scene where you must click on a poster with the word "finger" on it and obtain a finger of a dead man. However, this is only mentioned, not shown.

The methods of death in this game are very torturous in nature.

The first death in this game involves a character’s head being burned off at the neck. As of chapter 3, a character faces the risk of being incinerated inside a coffin.

A character commits suicide by cutting himself.

A character is crushed to death. You can hear and see it.

Kind of. A minor/background character is implied/stated to be pulled apart by chains in a victim video during chapter 2. The video is not optional, and critical to the plot of the chapter.

Kind of. One of the motive videos a guy gets squished

The father of one of the game’s participants (who’s also considered to be the “father” of the dolls) shoots himself in chapter 2

Some of the characters in this game are young children, and depending on the player’s decisions they may die.

All of the participants in the killing game have been kidnapped.

One character, Anzu Kinashi, is mentioned to work part-time as a clown and at one point shows her clown mask. It's very brief and not a major plot device.

Sara is stalked at the beginning of the game.

In a sense. The main character suffers scary hallucinations of her dead friends.

Optional bathing scene

Occasionally in the main character’s hallucinations her friend’s appearance suddenly changes to become terrifying. There is also one early in the game where the appearance of a character’s sister will also suddenly change to become scary while the character screams in agony.

no, but one does pee themself on screen

In chapter 1, a character is being reminded of a death and wets themselves.

(Optional) In the last fight of Chapter 3 part 1, a character has a PTSD flashback. If you make a specific choice during the fight, they will become triggered to the point where they throw up.

In one route, in chapter 2, a character undergoes something similar to cope with grief.

there is a medical room which some scenes take place in

There is a scene where characters are at risk of being injected with venom from a scorpion.

The main character has a few passing comments where they show insecurity about their weight/size, their weight is never disclosed and it’s nothing too serious

a character dies by cutting his wrists in chapter 1, and then in chapter 2 another character dies by shooting himself in the head

a character slits his wrists to avoid a worse death

The main character suffers several anxiety attacks over the course of the game wherein she hallucinates.

The dolls gaslight the killing game participants often, and Sou in particular often tries to derail conversations and arguments to make everyone else feel confused or question what has happened.

During Gin's memory, one of the antagonists, disguised as a teacher, says he can fix his autism if he signs a consent form.

it really depends on what is considered claustrophobic but a character locked themselves in a room and hid in a locker until they were found

There is only one black person in the game and he dies at the beginning. I'm not one hundred percent sure of the order but I believe he dies at the same time as other characters.

In a flashback scene a character is told by a major antagonist that he can be “cured” of his autism

the character Kugie Kizuchi has been confirmed to be lesbian or bisexual by a side game and she dies before the events of the game but her death is frequently mentioned (though she is not killed for her orientation)

One of the characters is an ex-cop and refers to himself as "Mr. Policeman" or "your friendly policeman". While he idolized a cop, the game itself displays the police, and especially one officer, as corrupt in later chapters. The two cops that were displayed in a good light both resigned due to the issues.

No one is misgendered, but a man character named Alice (who many interpret to be trans) gets teased about his gender

Several characters die. Depending on the player’s choices, the second chapter may have a highly upsetting ending.

It's possible for a character to be pushed down a deep hole into a pit of spikes, depending on player choices.

while there is no confirmed violence, it is heavily implied there is a former abusive relationship between two characters along with an unbalanced power dynamic. the abused character expresses clear discomfort when questioned about the other character, and depending on which route is chosen, this character must encounter their abuser.

one of the characters mentions being picked on by her older sister as a child to the point it might be considered abuse, another mentions having an alcoholic father who could be read as abusive

There is blood. The deaths are seen from a distance and the characters are pixelated, so it’s not too graphic in appearance.

Kind of. In chapter 3, a character can be pushed into a coffin in the ground, which is then crushed.

One character's backstory involves them shooting an unarmed person.

In chapter 3, failing an event will result in the characters drowning and getting a game over.

Although it is unclear whether she is being hung from her neck, a character's dangling legs are shown implying she was hung

a character has their ribs crushed to death in a sort of corset (if you choose this route, you can spare them of their death)