In Episode 130, Raxordal Uriela reveals he put together a boat for a cause, which included a "man so excited he brought his dog along with him." He was the only survivor, meaning the dog in question died off screen.
Momo's master, Orius Emoclew, was held hostage and got his fingers bit off every time the party attempted to contact him, which can be considered torture.
During the second City of Shimmering Lights arc, Korellian offers to sacrifice their own life to detonate the Kobold Oil Rig. They are resurrected at the end, but still tried to take their own life due to a belief that they were "living on borrowed time"
Considering how adventurer's usually go, they harm others and get harmed. While not usually explicitly described, it exists in the background (figuratively)
Dragotha (or something spelt similar) dies before the in-game events of FE, but Sindrae died and comes back to life sometime during FE, who later dies in episode 61.
Orius, Momo's mentor and the man who took him in upon his parents death (they're also part of this), died in episode 60 by Sindrae.
Trevlon's mother is also dead (died from a disease if I recall correctly), although she died before the in-game events of FE
In Episode 78, Quintessa and Skam use drugs, using Enhance Ability to replicate the effects. Skam believes that books can hear him, and attempts to burn a library
Although the fact it's kidnapping is found out much later, Orius (Momo's mentor) was kidnapped by someone working for Sindrae. This is found out during the Trapfest Arc
Yes, sadly Sindrae pulled a b***h manuever and killed two of The Party's horses, Horse and Pashen (I don't remember the episode but I wanna say around episode 57?)