One main character's story revolves around her rejecting her lesbian friend who has a terminal illness. The lesbian friend died before the show started, and her death is not shown on screen, but the main character is traumatized by her death.
Needles and syringes are used in a montage while characters are being treated for the Kiznaiver project, and are used periodically throughout in flashbacks.
In the second episode, several characters have anxiety attacks that are played for laughs when faced with their greatest fears. One character also implied to have PTSD, and has several panic and anxiety attacks when faced with their triggers.
In the first episode, a character is pushed down a very tall stairwell. The third episode features the protagonist jumping from an overpass, but surviving. Another main character repeatedly attempts suicide by jumping off tall structures. Finally, in the climax, two main characters fall off a tall bridge into deep water, but survive.
The main cast is put into situations where someone will get hurt, in order to test the Kiznaiver Project. The protagonist is repeatedly electrocuted throughout the second episode.