Drug use aside from alcohol or smoking is never explicitly shown but the plot of part 5 centres around a character becoming the leader of the mafia to get them to stop selling drugs to children
In Part 3, Jotaro is 17 years old, and several adult women find him attractive.
In Part 5, Fugo was implied to have been sexually assaulted by his professor when he was 13.
In Part 5, in Mista's backstory, a woman gets gangraped by several men. (Episode 8)
Also in Part 5, a stand tries to take Trish's top off.
The Baby Face Arc also has some non-consensual implications
In Part 7, an adult attempts to rape a child.
In Part 8 (During Paper Moon King), there is an attempt at sexual intercourse, not intentional rape due to a misunderstanding but clearly non-consensual. There is also another attempt in chapter 101
The part 4 antagonist routinely murders women and cuts off their hands, the part two protagonist looses a hand, and a character in part three looses a few fingers.
A villain is said to have cut a young boy’s genitals off after sexually abusing him. This is never directly shown however non graphic images of the police discovering what the villain did, are shown
In Stardust Crusaders, a character has their eyes clawed out in the desert. There is also a rather famous scene in Golden Wind where a character has his eye held open and burnt.
In Golden Wind (Part 5) Episode Notorious B.I.G. Part 2, Trish leans against a plane window and Notorious B.I.G. slams against the window from the outside
There are multiple, in fact. One involving the protagonist spying on their mother, there are also three others that involve both small children and murderers, simultaneously.
There’s actually a pretty consistent anti-cop stance in JoJo’s: part 2’s protagonist stops two policemen from brutalizing a young black kid, part 5 shows the rampant corruption in the police + justice system as a whole, and part 6 takes place in a prison so there’s plenty of anti-copaganda there. In other parts, police are shown either neutrally, or are just incompetent and actively impede the protagonists.
In Part 4 Yukako builds an electric chair, but it's never actually used. In the same part, the Stand Red Hot Chili Pepper uses Electricity to kill someone.
In Part 6, during the Dragon's Dream Arc, a character gets electrocuted. Same goes for the Highway to Hell arc. In Jail House Lock, a character repeatedly touches electricity
During part four we see a character be nursed back to health in a hospital bed after crashing their motercycle (Hands and feet are heavily bandaged). The mc heals their wounds before beating him up again (while said character is in the hospital bed). It’s shown in a pretty exaggerated manner in the same way most of the other scenes are.
Many of the characters who get into fights seem to have mental illnesses. For example, some protagonists of part 5 seem to have PTSD and regularly fight people. One villain seems to be mentally handicapped in some way and also regularly fights others
One episode in Part 3 involves the dream world and the real world interfering with each other as a minor antagonist can manipulate dreams of sleeping victims. In Part 6, reality becomes very unstable as two characters are dreaming while being melted by white acid.
Many of the characters display symptoms of PTSD however one very explicit character is Jotaro, who in part 4 seems to experience a flashback. The villain who he’s fighting resembles the one who caused his trauma both in appearance and at one point, manner of speaking. This causes Jotaro to fall to his knees before suddenly becoming violent
I’m the part 3 fight with wheel of fortune, the antagonist jokes about defeating jotaro and how part 3 is over now, when jotaro retorts with “so who will be the main character now” and then beats up the antagonist in a pretty amusing scene.
A woman gives birth in prison in part 5. There’s no nudity or graphic images of the birthing itself, however the woman screams in pain and bloody rags and water are shown in the aftermath
Only by technicality. In the manga, Adolf Hitler appears for a couple of panels, although he does not speak. Several other Nazis appear as minor characters, although no antisemitism is shown.
In part 2 (set in 1938), a very masculine dude dresses up in drag as a disguise to sneak into a nazi base. The nazis laugh at him but then get their asses kicked. I personally didn’t interpret the scene as anything too bad especially because the creator of the series is openly pro LGBT. But it does happen.
The English translation of the part 6 manga misgenders at least one trans male character. A minor trans male character is also held at a women’s prison. At time of writing, the part 6 anime has been announced, but it is not out yet. It is currently unknown how this will be handled in any language in the anime.
Two minor gay antagonists die in part 5, and the antagonist of part three dies and is confirmed to be bisexual, other than that there are a lot of dead protagonists though out almost every part who are often interpreted as LGBTQIA.
In part 2, a character insults a black boy in a racist manner. He calls him an animal, pig and says he has a foul odor. The racist man is quickly beat for his actions.
In the OVA, a dead womans breasts are shown. There is also a little girl in part 3 who’s butt is shown. Polnareff is also shown nude as a child in part 3. Uncomfy!
No, but Part 2's protagonist is shown to be attracted to his mother, and even spies on her in the shower. He is only made aware of this at the end of the part.
A bit complicated. In Part 4, a returning character from earlier parts is now 79 years old and is implied to be going senile. However, there are also implications that he’s faking it to mess with people, as he is a notorious prankster.
Santa does not appear at all, although he is jokingly referenced in part 3. It is not spoiled for children that Santa does not exist, although most kids probably shouldn’t be watching this program, as it’s rated TV-MA.
Parts 1 and 6 end with the deaths of the protagonists, and parts 2, 3, 5, 6, and 7 all conclude with the protagonist losing most, if not all of their friends.