The main character's husband is a Frankenstein-like scientist who experiments on animals. In the final scene, he grafts the legs of a calf onto the body of a dog. It is very obviously fake, but still can be very disturbing and upsetting.
Many animals are experimented on and abused. It is very difficult to watch, despite them being obviously puppets. The film's message is against this abuse, pointing out how some scientists view the furthering of mankind's technology to be more important than ethical treatment of animals and the environment.