In the first two minutes a bird is killed, and around an hour in a lot of birds get blown up. Both are incredibly graphic, and the second features lots of auditory screams that could be distressing.

Harley mentions that after a breakup her dog was killed

approximately 50 seconds into the film (when a character is bouncing a ball around his cell) a bird is killed and it’s quite graphic. further on in the film, a cage of birds is doused in fuel and then set on fire - you don’t see them burn, but you hear the screams and it’s quite distressing.

Several animals die on-screen. There are also some brief moments where animals are in “peril.”

In a flashback scene, someone is seen injecting heroin, and dying of overdose.

a character’s relative suffers from a heroin addiction, and in flashbacks (seen on the window of the bus) they can be seen injecting the drugs into their arm.

mild drinking scene in a strips club

King Shark eats people (not surprising) but one scene he coughs up a finger

A woman is suspended from chains and electroshocked; arm extension and toe curling is visible

in the opening fight scene a character burns to death, and there are close up shots of their corpse. later on, several characters are set on fire but that’s less graphic and less drawn out

there’s a quick shot where a character throws a weapon that lands in someone’s eye, and [SPOILER ALERT] the antagonist of the film has a giant eye that gets speared by an object and destroyed in a somewhat graphic manner

multiple nameless goons have their throats slashed by sharp weapons, particularly in an extended action scene with harley quinn. one of them is particularly graphic, as his neck is slashed almost the whole way through, and blood gushes out of the wound and his mouth before he dies.

A humanoid (but not fully human) shark character is shown eating people in a graphic manner

In the second half of the movie, when the characters step into the experimentation part of the tower, someone being experimented on is seen convulsing due to the link to the creature being tampered with. This shot is repeated two or three times.

in a fight scene, a character breaks someone’s arm (though it’s fast paced and there’s not much focus on it)

One character can remove their own arms at will. And does in the first few minutes

A lot! SPOILERS - mostly happens in the first 15 minutes or so on the beach. To start the fight on the beach Pete Davidson/Blackguard is shot in the head and his face explodes. This happens very suddenly. There is a shot of the corpse later. A soldier on the beach has their head sliced in half by a boomerang. Michael Rooker/Savant's head is detonated by a bomb. Later, a Starro victim is shown in the lab without the star and their face has been melted away.

A characters father ODs on heroine. It is briefly depicted

A family including children is shown hung with their faces covered

SPOLIERS Harley Quinn is taken after the beach raid by the "bad guys" and is trapped in a dirt pit for some time.

Harley Quinn in other media is depicted very clownlike, but in this she only has powdered pale skin and colored hair. Look up images of her character to see if it would be a problem for you but in most cases it would not be

A lot of crowd extras get controlled by a monster

gunshots act as jumpscares throughout the film

A fair amount of characters spit up blood or water

The entire movie features heavy audio gore, including but not limited to; animal death, breaking bones, amputation, blood splatter, bodily explosions.

Polka Dott man (whatever he is called I can’t remember exactly) gets up in the middle of the night and walks off to vomit behind a bush. It’s barely visible and doesn’t look like vomit anyway, I wasn’t even sure he was being sick at first. Just watch out for the scene where he wakes up in the night and has the spots on his face if you’re worried about it.

not sure if this fits the category, but a character is tortured with an electric rod and shocked several times by it

[SPOILER ALERT] In the second post credits scene Peacemaker is shown to be lying on a hospital bed in a state of comatose as a nurse tells a couple of Waller's agents that he will recover soon

syringes are used to inject devices into character’s skulls, a character suffers from a drug addiction and is seen with a syringe in their arm, there is a javelin used as a weapon

Polka Dot Man is shown vomiting once or twice but this is specified to be due to a virus affecting him.

In a sense, it's the entire plot of the film. They're on a suicide mission. Many die. One character even mentions wanting to die before going on the mission.

There are no specific anxiety attacks but there are multiple scenes where characters experience extreme panic, including yelling, hyperventilation and thrashing.

No characters are gaslit, but in keeping with the first movie the character 'Amanda Waller' is extremely manipulative and immoral. She directly manipulates a character into obeying by threatening to have his daughter placed in prison, vagueing that she can orchestrate her death.

Not outright; but one character says “I hope so” after another says “we’re all going to die.”

Described only, one character discusses having been locked in a small crate with hungry rats for 24 hrs as a child

Opening scene includes a camera manipulation where the entire image spins and gives a vertigo effect. I watched it in imax and it made me a little nauseous

Gunshot effects cause some blinking lights throughout the movie.

There is a background character who’s penis is very visible. It is not sexual.

In the third act of the movie when all of the heroes are seeing Project Starfish for the first time, Starro (speaking through it's human hosts) tells the Squad that it has been tortured, taken advantage of, and uses the phrase about its captor, "he had his way with me." Grievous makes a face that, in my opinion, confirmed that he most likely raped or sexually assaulted the human hosts, and therefore Starro. It's only implied, but I believe it to be triggering in the way it's phrased and commented on by the characters.

In the bar scene when it focuses on Polka Dot Man dancing, it shows his point of view where he’s imagining all the girls dancing around him as his mother.

“Mental defective” and “crazy one”. It’s not used super seriously but it’s there

There is a brief mention that the main villain's hub was once used for Nazi experimentation and that the Nazis were protected there after WW2 before being killed eventually when the price to kill them became higher than the price to keep them alive.

[SPOILERS] Polka-Dot Man is openly interested in men and does not make it out alive

Not necessarily sad, but multiple characters die by the end, making it very bittersweet despite the team’s “success” on the mission

Helicopter crashes in first 5 minutes

There are many scenes of characters falling off buildings or through holes/pits. They do not die but for a moment, the audience believes they might be dead

No, but Harley does mention having been in abusive relationships.

There are mentions of past child abuse but nothing is shown

This is an *extremely* gory movie, there are many, many graphic on-screen deaths, including bodies exploding, being ripped apart, cut in pieces, eaten by King Shark, shot to death, etc, and the camera often focuses on corpses in various awful states of death

Not buried alive but a character is temporarily kept in a hole dug into the ground.

The majority of the movie heavily features gun violence, including injuries, deaths and audio gore.

No but there are many small explosions.

Weasel drowns but does not die.

An entire family is shown hung with their faces covered including a child

There are scenes of choking and drowning.

towards end an extra is hit by a car but only seen for a few seconds