Graphic dog death. Do not watch the final segment (the one with the teenagers and kids having a sleepover) if seeing a dog get hurt and then die would be too upsetting. None of the other segments have dogs in them.
It's not explicitly r*pe, but there is a shot in the Indonesian segment where a woman is being penetrated with a sharp object during intercourse. It takes place in a cult's commune where its members are willing to carry out horrible acts, but also where regular sexual assault of minors is heavily implied.
On the contrary, in the last segment, a family running from murderous extraterrestrials makes sure their dog is not left behind, even often putting the pet before their own lives. Sadly, everyone--dog included--perishes in the end. (About 1:26:00-1:27:00 mark)