Graphic dog death. Do not watch the final segment (the one with the teenagers and kids having a sleepover) if seeing a dog get hurt and then die would be too upsetting. None of the other segments have dogs in them.
It's not explicitly r*pe, but there is a shot in the Indonesian segment where a woman is being penetrated with a sharp object during intercourse. It takes place in a cult's commune where its members are willing to carry out horrible acts, but also where regular sexual assault of minors is heavily implied.
On the contrary, in the last segment, a family running from murderous extraterrestrials makes sure their dog is not left behind, even often putting the pet before their own lives. Sadly, everyone--dog included--perishes in the end. (About 1:26:00-1:27:00 mark)
The Slumber Party Aliens segment is full of children and teenagers using vulgar and profane language (because they're kids and there's no grown ups around.)
Only once in the Slumber Party segment, a teenage boy calls other teenage boys the f slur. Once again--just immature kids being foul mouthed, nothing that harmful.