It's not directly BDSM/kink-related, but in the fourth case of the second game a situation is described with one character using collars and leashes on people, so parallels could be drawn to BDSM/petplay
In "The Adventure of the Clouded Kokoro," a woman verbally abuses and throws things at her husband. The abuse is played as comedy and is involved in the solution of the mystery.
In cases 2-4 and 2-5, a character is shown to have repressed memories and confusion around an incident in the past. At times, another character who held a position of power over them at the time tries to cover up what really happened by arguing against the former's memory of the events
The main prosecutor is often shown pouring wine, but he rarely drinks said wine, opting instead to throw the chalices or the bottle to emphasise his points.
One of the jurors in "The Adventure of the Unspeakable Story" has an animation where he squeezes a mouse. The mouse looks uncomfortable but is unharmed.
Some locations throughout the game have decorative taxidermy mounts that can be examined. In "The Resolve of Ryunosuke Naruhodo," there are mentions of a character's pet dog dying several years ago.
2nd chapter.
Spoilers: there's one static image of a snake in the intro cinematic for the 2nd chapter.
Later in the chapter, one of the characters have a big snake coiled around its head. It moves and make hissing noises. It stays there for a while and is visible during many lines of dialogue, including a "deduction" mini-game.
Several characters in Case 1-2, "The Adventure of the Speckled Band," have a soporific slipped into their food before the main events of the case. It has no adverse effects besides complaints of headaches, but it is a relevant plot point that the protagonist mentions quite a few times.
While not depicted, there is discussion of relevant backstory involving a serial killer whose victims are described as having their throats ripped out.
A character in "The Adventure of the Clouded Kokoro" and "The Memoirs of the Clouded Kokoro" describes struggling to breathe at night due to gas leaks in the room he rents. The culprit's breakdown animation in "The Adventure of the Unspeakable Story" involves them trying to strangle the witness next to them, who is shown gasping for air.
Someone burns to death inside a carriage at the end of "The Adventure of the Runaway Room," the inside of the carriage isn't shown but the person inside is heard banging on the locked door. In "The Resolve of Ryunosuke Naruhodo," the culprit's breakdown animation involves them standing in front of a wall of fire, and their character model has cartoon singe marks afterwards.
Part of the backstory of "The Return of the Great Departed Soul," "Twisted Karma and His Last Bow," and "The Resolve of Ryunosuke Naruhodo" involves a person being buried alive. There aren't POV visuals, but there's multiple images and a sculpture of the person emerging from the grave they were buried in.
In "The Return of the Great Departed Soul," the detached arm of a wax statue is shown. Later, the rest of the statue's body is seen, with a visible stub where the arm was removed. Another statue is seen without its head. A character who appears later in the chapter wears a gauntlet that looks like a prosthetic hand.
In "The Adventure of the Unbreakable Speckled Band" and "The Return of the Great Departed Soul," the victims are determined through postmortem examinations to have broken their necks.
In "The Adventure of the Unbreakable Speckled Band," the victim is determined to have tripped and broken their neck. In "The Return of the Great Departed Soul," the possibility of the victim being killed by a fall rather than the stab wound listed on the autopsy report is briefly discussed. In "Twisted Karma and His Last Bow," a character attempts suicide by jumping out a window and survives.
A teenager stands trial for murder, a capital offense, in "The Adventure of the Unspeakable Story," however the objective is to get them acquitted. Even if the player fails, you never see your client executed in any case.
The victim of case 1-1 is a parent of a major character. The victim of case 1-3 is a father, and 2-4/5 is a father figure to another character. There are also references to orphans and parents having died in the past.
In "The Adventure of the Clouded Kokoro," a woman accuses her husband of cheating after finding a love note in a used book he bought that the book's previous owner was using as a bookmark.
In "The Adventure of the Clouded Kokoro" and "The Memoirs of the Clouded Kokoro," a character rents a room that's rumored to be haunted by the ghost of a former inhabitant due to frequent gas leaks.
For someone jumpy and unfamiliar with Ace Attorney style, some of the movements and sound effects may appear sudden, so bear that in mind as these happen consistently.
Debatable. There are police and police detective characters who are portrayed sympathetically, even after tampering with evidence or using other underhanded methods in some cases. At the same time, there is explicit criticism of the police force and intrinsic corruption in law enforcement and the judicial system is a major theme.
In "The Return of the Great Departed Soul" and "Twisted Karma and His Last Bow," syringes can be seen in the background along with various other surgical tools while visiting the coroner's lab, but they aren't used and can't be examined or interacted with. In the extras section, the concept art for the coroners Dr. Courtney Sithe and Maria Gorey includes a sketch of syringes.
One character is caught attempting suicide by drinking poison, but she does not drink it and stays alive. Another character attempts suicide by jumping out a window, but he survives.
A character who appears in "The Adventure of the Clouded Kokoro," "The Adventure of the Blossoming Attorney," and "The Memoirs of the Clouded Kokoro" frequently starts shaking from anxiety and paranoia. A character in "Twisted Karma and His Last Bow" sways awkwardly and holds his head as if in pain while trying to remember a traumatic event, then screams and passes out upon remembering it. Throughout the game, multiple characters have "breakdown" animations when cornered while testifying.
In "The Adventure of the Unspeakable Story," a character regularly holds a loaded gun to their head and threatens suicide as penance for doing their job poorly, played as comedy. In "The Memoirs of the Clouded Kokoro," the player interrupts a suicide attempt by drinking poison. In "Twisted Karma and His Last Bow," a character has a flashback to a suicide attempt by jumping out a window that they survived.
Characters are briefly seen in confined spaces or describe being in a confined space in "The Adventure of the Unbreakable Speckled Band," "The Adventure of the Runaway Room," "The Return of the Great Departed Soul," and "The Resolve of Ryunosuke Naruhodo." There are no POV visuals and the characters don't show distress.
A character in "The Adventure of the Unspeakable Story" frequently holds a loaded gun to his head while threatening suicide as penance for failure, played as comedy. "The Adventure of the Blossoming Attorney" ends with a character implying that they're going to perform seppuku ritual suicide but instead they cut off part of their hair. A character in "The Memoirs of the Clouded Kokoro" attempts suicide by drinking poison but is interrupted by the player. In "Twisted Karma and His Last Bow," camera pans and zooms are used to imply that a character attempted suicide by jumping out a window and the survived attempt is mentioned occasionally afterwards. Suicide is occasionally brought up as a possible cause of death in multiple chapters.
A character in "The Return of the Great Departed Soul" had their hair turn white after a traumatic incident. A character in "Twisted Karma and His Last Bow" repressed their memories of a traumatic event.
Ace Attorney as a series sometimes uses sudden sound effects to represent different kinds of sounds. Someone might not like them if they are particularly jumpy
The screen flashes and shakes slightly when characters make important points during trials. In "The Adventure of the Unbreakable Speckled Band," it shakes when the ship the case is set on makes an emergency stop. In "Twisted Karma and His Last Bow," it shakes while the POV character is being attacked by a puppy.
Investigation segments are presented in a first-person view from the player character's perspective. Characters sometimes interact with the screen as interactions with the point of view character. There are no points where characters address or interact with the player as the player.
There aren't any depictions of antisemitism towards characters established as Jewish. However, a predatory loan shark character in "The Adventure of the Runaway Room" is referred to as "a Shylock" at one point, referencing the antagonist of the Shakespeare play The Merchant of Venice, who is widely regarded as an antisemitic caricature, has a history of being used in antisemitic propaganda, and whose name has been used as an antisemitic slur.
"The Adventure of the Unbreakable Speckled Band" and "The Adventure of the Blossoming Attorney" both involve female characters disguising themselves as men. Accordingly, other characters refer to them as men and use he/him pronouns for them while they're disguised.
"The Adventure of the Clouded Kokoro" involves two fat women whose size is frequently drawn attention to in an unflattering way, with a handful of jokes at their expense. One of them returns in "The Memoirs of the Clouded Kokoro," and her size is drawn attention to again, though not as much.
There is a lot of casual anti-Asian racism against the main character and other characters in the first case by a white antagonist. Some characters disregard or encourage it, but it is ultimately framed as wrong.
Gina Lestrade, a major character who is a pickpocket and describes being an orphan who lives in the East End of London, with no one to rely on. You never see her living conditions, but she sometimes describes them. She comes into the care of a police officer in the second game.
The departure and separation of main characters is present in the endings to the first and second games, however this is ultimately framed as hopeful with promises to meet again.