There is a scene when Anna is exiting the metro where she begins to thrash around that ends in an upsetting scene where she seems to have a sort of seizure and excretes a foamy liquid from her body.
(SPOILERS) Hienrich's mother takes poison pills to kill herself after she realizes Hienrich is dead.
Also, Mark jumps to his death after being shot multiple times. He would have died from the gunshots anyway.