The anime is about a videogame world. There's plenty of violence but wounds are represented by the affected areas of the body disintegrating into small shiny square particles
The post-credits scenes address the audience directly, presenting a bonus scene, an outtake, or a joke based on the episode just seen. S01E14.5, a recap episode, has no fourth wall at all, referring to individual scenes and episodes as such and critiquing each others' actions.
S01E12. A male player is running a female character in the titular MMORPG. When an NPC refers to him as "her," the protagonist gently corrects her mistake, describing the situation as though the female character is a pre-transition trans man. The NPC accepts it, but is utterly confused because they never thought of that as possible. The only part played as a joke is the NPC's reaction at the end.
Played for "laughs." The protagonist claims to have been traumatized by a badly-programmed dating sim and to have PTSD flashbacks when he has to juggle NPCs' opinion of him. He stands motionless and trembling at the thought.