A few of the player characters (Shrimpo, Scraps, Pebble, Flutter) are based on animals and can be killed in-game. Most are anthro except for Pebble (and arguably Flutter? maybe)
One of the playable characters, Scraps, while technically not a cat is still very visibly catlike and (like all other playable characters) can be killed
An audio stinger when a twisted spots you, a barking sound when Twisted Pebble spots you, a buzzing sound when Twisted Vee spots you and a glass shattering noise when either Twisted Rodger is activated or when Twisted Glisten goes berserk (happens either when the last machine is finished or when someone leaves him alone for too long)