In one route, the protagonist is haunted by an invisible spirit who repeatedly makes noises such as door knocking and footsteps throughout the protagonists room for several minutes straight. It does this multiple times.
Shouma's Dog Leo was abandoned, though unintentionally on his part (he was kidnapped and drafted into the titular academy). He mentions frequently to wanting to go home for his dog.
Several characters, including the protagonist, are having their memories tampered with so they can be gaslighted into believing things that aren’t true.
One of the routes has a pretty blunt drug addiction metaphor where the protagonist is chronically depressed and becomes extremely dependent on a “motivational gadget,” which makes the protagonist act uncharacteristically over-the-top energetic constantly and causes them to black out for several days and lose memories.
In one route, most of the characters, who are all underage, get drunk to cope with the grief of a friends murder the previous day.
In another route, the protagonist (who again, is underage) is tricked into consuming a glass of high-strength alcohol. He quickly passes out then experiences a painful two day long hangover.
A character repeatedly takes a drug that basically forces everyone around them to fall madly in love with them, even if they have zero interest in him.
In one of the endings, a character is drugged to be in a permanently vegetative state where they are conscious, but completely paralysed and unable to move or speak. The game ends right as they are about to be raped while stuck in this state.
A character gets high on sleep medication one night, sends everyone satanic messages in the middle of the night and forgets all about it by the next morning.
Also one of the routes has a pretty blunt drug addiction metaphor where the protagonist is chronically depressed and becomes extremely dependent on a “motivational gadget,” which makes the protagonist act uncharacteristically over-the-top energetic constantly and causes them to black out for several days and lose memories. The ending implies their addiction gets worse after they confess to the girl they like and she says she doesn’t like him as a person. This is all played off as a joke (The route this all takes place in is called “The Comedy Route”) but it’s still rather morbid.
No animals at all are shown throughout the entire game with two exceptions. Firstly, deadly robotic bees. Secondly, in one route an unknown animal (not a cat dog or other pet like creature) died and is infected with a parasite.
It's not actually "on-screen" like it would be in an eroge (more of "fade to black" kind of thing), but it is very clear what happened and very clear a character couldn't adequately consent. It's in endings 41 and 42.
It’s stated that Ima (a middle school boy) has had to work several “underground jobs” in order to earn enough for him and his twin sister to survive as homeless orphans. It’s heavily implied that one of these jobs included prostitution.
This is a reoccurring theme throughout the Cult of Takumi Route as you’ll notice in the other rape/sexual assault categories. The choice that leads to this route isn’t obvious at all so for those who want to skip it:
When you are given a choice to sacrifice one of three people DON’T sacrifice Takemaru. This is the only route this choice leads to and you don’t miss out on any important plot points for skipping this route.
One of the main characters is a “mean girl” archetype whose also a trained boxer. In several routes, she beats up another character for really petty reasons. In the first playthrough route, she sets up a fight against the smallest and physically weakest person in the entire group.
There is a sprite of one of the characters with their eye visibly injured and bloodshot. In one route, a character rips off their own eyes, then steps at them. The process isn't shown, but their ripped off eyes can be briefly seen on a CG.
In one of the routes, the main characters are captured by the antagonists and interrogated for information. In one scene, the protagonist see’s the main villain arrive covered in blood after torturing one of his friends but who it was and how they were tortured is left ambiguous.
There is a character with a torture fetish who makes multiple graphic references to acts of torture.
Also a character absorbs a parasitic organism and lives in constant never-ending agony from then on.
In the first playthrough route, an enemy uses shape shifting powers to disguise itself as a person and infiltrate the academy. Their form begins to fail, and their body decays into a grotesque form of melting skin.
In another route, a character absorbs a parasitic organism and their body gradually deforms into a lovecraftian-esk monster.
The protagonist gets decapitated in battle (though he's revived shortly afterwards) and his head gets cut off with a butcher knife in one bad ending. Another character also decapitates themselves with their own weapon after the protagonist tries to spare their life (the moment is not actually shown, but the CG + the sound effect are very telling). In other route, the character also offers a bag of cut off heads of the enemies to prove their loyalty.
A character has their throat slashed and you can see them struggle for a bit before they die. Similar thing happens to the protagonist in few bad endings.
The protagonist is choked until they fall unconscious in one route.
In one of the endings, the protagonist has a chain wrapped around their neck by the main villain, and is dragged by the neck throughout the building as the villain slaughters all of the protagonists friends.
The thing the students are protecting in the defence room is revealed to be a baby inside a machine with supernatural fire powers. The baby is murdered to steal their powers in several routes, including the first playthrough route.
The characters have access to a machine that revives them if they are brought there soon after death, so there are numerous instances of characters dying and then being revived. However there are circumstances where characters can die off permanently due to being unable to access the machine fast enough for one reason or another.
Not only that, but often you are forced to make a decision where you can only save one person and must leave another person to die, or whether you have to kill certain main characters.
There is a character who is an adoptive mother who dies in most of the games endings.
Several instances of offscreen parent deaths prior to the games events; Nozomi’s mother committed suicide when her life’s work was shut down, One of the main villains states that they murdered their own family, A little girl’s parents are murdered in front of her for opposing a military dictator.
The game opens with all of the main characters being kidnapped and essentially drafted into a war they have almost zero context on and the little information they are given is untrustworthy.
It’s revealed in some of the routes that the protagonist’s brain is being controlled by a parasitic organism with human level intelligence, which has hijacked his body and forced him into committing serial murders.
In another route, the protagonist is possessed by one of the main antagonists who intends to fully take over the protagonists body and murder all of their friends. Regardless of your choices in this storyline, it always ends with the antagonist winning in some way, whether it’s because he fully takes over the protagonist, tricks him into killing all of his friends himself, or drives him into insanity for the rest of his life.
In another route, a character willing absorbs a parasitic monster into their body in the hopes of controlling its revival powers. As time goes on, the creature possess them more and their personality begins to regress and their body begins morphing into a lovecraftian creature.
There’s a reoccurring ghost-like character that follows the protagonist throughout the game. The protagonist assumes them to be hostile initially but it’s later revealed that they are friendly.
In one route, the protagonist is haunted by a ghost which is initially implied to be the vengeful ghost of a woman begging to be spared that he murdered, but is later revealed to actually instead be a manifestation of his guilty consciousness over killing this woman. The protagonist lets this ghost kill him out of guilt in two of the endings.
A main character has a medical condition where they vomit whenever they suffer from high stress. They make frequent retching sounds throughout the game.
In one of the routes, A woman kisses the protagonists girlfriend for close to a full minute and the woman later jokes that she cucked him to try and anger him.
In one route, you are forced to perform electro-therapy on an enemy commander multiple times to “correct” their personality from who they once were into something Sirei deems acceptable. Each time the resulting personality is deemed unsatisfactory by Sirei, you are forced to do it again the next day until he deems it as properly corrected. This is depicted as a horrific thing by the narrative, but the fact that the player is forced to press the button themselves makes the entire sequence extra uncomfortable.
A couple endings have characters, including the protagonist, committing suicide out of depression.
In one of the endings, the protagonist commits suicide literally hundreds of times (for context: There’s a machine that revives him over and over again) to try and get rid of someone possessing him. He does this through a variety of methods which are all described in graphic detail including drowning, hanging, stabbing himself, jumping off a roof and inhaling car exhaust fumes.
Characters stab themselves in the chest with a knife as a part of their combat transformation. One of the route includes the protagonist self-harming and killing himself (though he knows he will be revived) repeatedly in increasingly painful ways to achieve a specific goal. In another route, the protagonist's guilt causes him to choke himself during a dissociative episode.
It’s never explicitly stated to be due to an eating disorder, but Darumi is stated to seldom eat any proper meals outside of snacks, despite having constant access to a machine that instantly makes any kind of meal automatically.
One of the commanders abilities traps the characters in what’s basically a dating simulator game with a drastically different art style and tone from the rest of the game.
In one ending, the protagonist hallucinates the halls covered in gore, deceased characters being alive and stops having any significant reaction to seeing any more of his friends corpses because he can’t tell anymore when he’s hallucinating or not.
In another route, the protagonist is brainwashed into living in a delusional world, where nothing bad happens, nobody actually died and all his problems are mundane. Eventually he recovers his memories, realises he’s trapped in a delusion and has a mental breakdown. He can then choose to spend the rest of his days living in a happy delusion or recovering and experiencing his death at the hands of the main villain.
In another ending the protagonist is killed by his love interest, who is suddenly acting maniacal, wakes up the next morning apparently alive, acts unnaturally cheerful for a week, the enemy leaders react uncharacteristically terrified of him, and then he sees his own corpse on the floor before the credits play.
It’s revealed during the first ending that the main villain was secretly watching a private conversation between the protagonist and another character, who later spreads this private information to everyone else to turn the group against that character.
Multiple fourth wall breaking jokes. There’s also a joke route where Sirei says he has run out of ideas for different endings, randomly decides to blow everyone up, all the other characters complain about how terrible this ending is, and Sirei directly speaks to the player telling you to “Die mad.”
If at the start of the game, you refuse to fight, you will get the early bad ending. A character will tell you “you better not leave a bad review complaining the game is too short.”
Vague. One of the endings leaves off with just the protagonist and a pregnant woman left in a destroyed world where they are being hunted down by the surviving inhabitants. It’s left ambiguous how long they manage to survive for after the fact.
The thing the students are protecting in the defence room is revealed to be a baby inside a machine with supernatural fire powers. The baby is murdered to steal their powers in several routes, including the first playthrough route.
It’s revealed in the true ending that all of the main characters, excluding Nozomi, are actually all artificially created people from a single persons DNA, effectively making them all siblings. In some of the other routes, the characters can enter relationships or engage in romantic acts with each other while being unaware of this fact.
I don't think you actually see anything graphic (covered by hair iirc) but the Supreme Commander strips and swims in the pool in front of Takumi in one route
There’s a scene where a brainwashed woman proves she will do anything she’s told by stripping her clothes off. This is portrayed as unnerving but one of the characters has an aroused reaction which is played off as a joke which needlessly muddles the tone of the scene.
The Cult of Takumi routes features basically every character being drugged into performing sexually objectifying acts (both the men and women).
The main selling point of the game is the fact that there are 100 endings. All ranging from Comedic, Happy, Sad, Horrifying, Vague etc. There is technically a “true” ending (The one labeled ingame as Ending 01) which is a really sad ending, but the developers have also said it’s up to the player to pick which ending they want as their own “true ending” to the game. So really, it’s up to you.