Frequently. While some characters' mutations simply turn them into bipedal animals or objects, many mutants are horrific in appearence and seem to be in pain. Special mention goes to Melting and the Freak IDPDs.
The police (IDPD) are an antagonistic force that attacks the player on sight, unprovoked. While their goals are somewhat up to interpretation, they seem to be selfish, and even if they weren't it would not justify the means.
A few of the mutations cause lip-licking sounds when selected, and Mimics lick their lips in place of the normal chest's shining animation. A few of the songs also have breathing sounds.