A puppet dog is alluded to being "killed" when the main antagonist manipulates one of the other antagonists, this happens again when he threatens to blow up Roscos' brain. Very triggering if you are sensitive to this.
Owen Gubberson is abused by his father. All of the puppets are abused by the main antagonist in almost all ways, and one of the recordings that can be found around the studio features verbal (raised voice) and physical (the sound of a slap/punch on wood and a character asking why he was hit) abuse.
Main antagonist mentions a medieval torture instrument (the rack) and another character panics as he is presumably dragged away. This all happens in audio with no visuals of the incident whatsoever.
No romance, but Nick Nack is implied to have borderline incestuous feelings towards Riley Ruckus who is later described to have a siblinglike relationship with him. Fandom mostly ignores this, the feelings are not reciprocated and it is treated as an abberation.
this is mentioned and a character is hit with what sounds like an open palmed slap. There is no visual but a loud wooden sound and reaction to being struck during one of the security tapes. The same character who was punched mentions being beaten ten times more than any of the other puppets, giving a very loose description of presumably physical abuse.
Not by other characters, but borderline does so to himself. A male character is heard crying but the player character is shown to be concerned about it. When Owen Gubberson asks Nick Nack if he's crying, he shouts 'GENIUSES DON'T CRY!' which can be classed as a negative reaction.