While not joked about (and are referred to as beautiful), there are some characters that are gender non-conforming women who unintentionally can be seen as transmisogynistic in design.
Does not happen on screen, but one main character mentions that their parents left them from a young age before they could remember, mentioned in the first half of Act 5. The protagonist is also mentioned to not know their father's face in the epilogue, implying another case.
There is a pregnant woman, and her future child is referred to. In addition, one of the developers put in his commentary that he has a child on the way as well. There are no on-screen babies.
A character refers to the controller you're using for a dance tutorial. At the end of the game, there's a secret talk with the developers where they break the fourth wall to talk about what making the game meant to them.
The closest thing in the game is some mermaids who have gotten tired of being asked for magical favors, etc. A man meets a mermaid for the second time and asks to marry her, for instance. However the art style is very cartoony and I would say they're very tame. Other than them, this game has very cool and properly dressed women.
A character has self esteem issues that cause her to almost end the world. She's not depicted as anybody but a sad person who made a bad decision, though.
There's a puzzle later in the game that uses little bugs that group into a mass to overcome physic challenges. The artwork of the game makes them very tame, but it's possible to feel a little squeamish.
There is multiple scenes where your character is used to convey information from deceased spirits to their family or friends. Sometimes upsetting but never malicious.
There are a lot of ghosts, and most of them don't have specific faces or races. However, one of them is of a young black girl. She's very plot important.
The closest the game gets is something acknowledged in a bit of developer commentary. One piece of background music happens to be 69 seconds long, and a relevant character will says "nice!" once it ends.