2 characters in part 5 are rumoured (And heavily implied) to be a gay couple. One is tortured and killed while the other is made to watch, later killing himself. They are mostly mentioned during a flashback and this is prior to the main story. A few other character are said in interviews to be lgbt (like Dio being Bi) but it’s very rarely mentioned in the series
Very rarely in a haunting sense but stand users tend to have a sort of angelic spirit appear for a few seconds after they die. (SPOILERS) in part 4 a ghost is met who was stabbed to death as a child by a serial killer who is the main antagonist of said part
As previously described there's a scene in which a character goes in drag to sneak into a nazi base, while the nazis immediately see that character as a man and treat him as someone trying to trick them (which is accurate as that's exactly what he tried to do) it is not treated like "traps" commonly found in fiction, and as a trans woman myself there's no reason to be offended by it
I suppose. In part 5, a character called meloné is seen licking their lips whilst looking at a woman’s legs, after which the woman is impregnated offscreen.
Additionally to the nazis there is a racist who wants to get a black man kicked out of a restaurant, but this is not endorsed by the story or any of the other characters and he is beaten up later