Yes; Issue 5 of the Universe series features a dog fighting ring. We don't see any actual fights, but there's a kidnapped dog being dragged in and an injured/dead dog being carried out.
Technically; a character commits ritual suicide with the intention of later being resurrected as an immortal. In a vision, several people leap to their deaths after being put under an evil spell, but this event is prevented.
Yes; the words "psycho" and "freak" are used often, and one villain refers to a disabled person's life as "meaningless" and another villain wants his deformed son to "look normal". Additionally, there are some ableist writing tropes- at one point a healing serum is used to "cure" a disabled character (a stroke survivor who was a wheelchair user) but also positive rep such as Donnie's prosthetic shell.
Yes; minor villains sometimes make gross comments about female characters, other character jokes about their friend "getting some action" with his girlfriend and said friend is outspokenly uncomfortable with it
Yes; There is a romance between an immortal being and a mortal man. They are both adults, but it's a little strange because the immortal both knew and was in a relationship with the moral character in his past life as well. "Romance" used loosely, however, as the immortal being doesn't really care for him, and is just using him.
Yes; characters are ostracized, demonized and dehumanized for being mutants. Anti-mutant sentiments are spread online and through reactionary news outlets. It might hit close to home for those who have faced similar oppression.
Techinically no; A character talks about ending their life, but this is an internal monologue. Another character discusses their plan to explode themself, and later begs another character kill them. Another character says "I'd rather be dead than the way I am now". However, this exact phrase is not used.
Yes; at one point in a flashback through time, we see a N**i soldier. Additionally, the Rat King is drawn in a way that resembles antisemitic caricatures. There is also discussion of antisemitic conspiracy theories like secret societies and blood libel (although there are mostly portrayed by villains).
Partially; One character stands in front of a mirror and harshly tells himself to stop crying. Another character is told by his mother (who is villain) to stop crying, but only briefly.
No; in the 2021 annual, it appears as though the Rat King is talking directly to the reader, but this is just due to the framing (we don't see who he's actually talking to until the end)
Yes; The spirits of dead characters appear sometimes in dream sequences. We see an afterlife setting where there are wandering spirits as well. And, of course, there are ghosts in the Ghostbusters crossover.
Yes; In the Ghostbusters crossover, one of the characters is possessed by an evil spirit. Also, one of the main villains has a mind control power that works like possession